2. Auxin resistant mutants

Seven plants growing on media supplemented with 50 uM indoleacetic acid (IAA) were selected out of 7,000 screened. This concentration of IAA inhibits the growth of WT plants (Mirza et al. 1984). Of the seven putative mutants, six retained the auxin resistant phenotype in the next generation and were further characterized. All six mutants had longer roots than WT plants in the presence of 50 uM IAA (Figure 2.1). Interestingly, of the six mutants only one, JKS-6, displayed ageotropic root growth in the presence of IAA. Also, four of the mutants had longer hypocotyls than WT plants in the absence of IAA (Figure 2.2). Results suggested that the mutants were not all identical, and that the mutations affected growth and development differentially reflecting complex patterns of gene expression.


  • James Madison University, Department of Biology
    Jonathan D. Monroe, PhD
    Comments to author:
    monroejd@jmu.edu

    All contents copyright (C) 1995, Jonathan D. Monroe. All rights reserved.
    Revised: December 22, 1995
    URL: http://csm.jmu.edu/biology/courses/bio455_555/atlab/aux.html