week 1 - Introduced project (see Appendix 3), surface sterilized mutagenized seeds, and plated them on 0.5x Murashige and Skoog media supplemented with 1% sucrose and any additional compounds to use for mutant selection.
week 2 - Looked for mutants and transplanted them to soil in 5" pots subirrigated with nutrient solution. Grew plants at about 24 deg C in a 16/8 hour light/dark cycle. Shorter photoperiods delay flowering.
week 3 - Looked for more mutants as needed and transplanted.
weeks 3 through 8 - Conducted traditional labs from "Experiments in Plant Physiology" (Carol Reiss 1994). I chose labs that some of the students could repeat later with their mutants.
week 7 - Harvested seeds when siliques started to turn brown, dried in 9x12" paper envelopes at 30 deg C for one to two weeks (test germinated some seeds on plates after one week).
week 9 - Cleaned seeds. Surface sterilized and plated putative mutant and wild type (WT) seeds under the same conditions as the mutants were isolated to determine whether they were really mutants.
weeks 10-15 - Conducted experiments with known mutants. Students wrote a short group proposal prior to starting their experiments.
week 16 - Held symposium where each group presented their results to the class. Turned in group papers written in the style of a manuscript to be submitted to the journal "Plant Physiology".
All contents copyright (C) 1995, Jonathan D. Monroe. All rights reserved.
Revised: December 22, 1995
URL: http://csm.jmu.edu/biology/courses/bio455_555/atlab/schedule.html