Molecular Biology 580 Requirements
Additional Lecture Requirements: In addition
to completing the BIO 480 lecture schedule, BIO 580 students are
required to identify a topic in Molecular Biology agreeable with both
the student and instructor before Spring Break (March 11). The
student will do research and use primary research articles to put
together a 30 minute talk to be given during the last week of class
on the topic and turn in a list of resources to the
instructor.
BIO 580 Laboratory Schedule
The green highlights indicate experiments that differ from the BIO 480 course. In the second half of the semester BIO 580 students will be expected to observe what BIO 480 students are doing however grants for microarray slides do not allow graduates to work directly with the slides. The BIO 580 students will be expected to work independently on labs and put together a presentation comparing the microarray technique with the technique of using RT-PCR. What are the advantages and disadvantages of each technique?
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Isolate clones from the Arbidopsis cDNA library |
Isolation of Clones Culture Preparation |
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(the day before lab) Preparation of plasmid DNA from clones |
Plasmid Miniprep Exploring Databases |
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Agarose Gel |
Restriction Digests |
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Preparation of DNA Sequencing Gel |
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Editing your DNA Sequences Data Base Information Accessing and Understanding Sequence Databases |
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No lab on Feb. 18 Assesment Day |
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Growing Yeast/ Isolation of RNA |
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Analysis of RNA |
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Clean-Up |
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Go to Course Home Page
Go to Laboratory Home Page
March 24, 2003
T. Rife