This  earlier lab manuals are not intended to represent a complete, self contained lab course. The overall idea of the project is that students pick anonymous cDNA clones from a rescued library and then spend the entire semester characterizing, sequencing, identifying, and subcloning them, then sequencing some more. We find libraries constructed in the pZIPLOX vector from Gibco BRL to be convenient since rescued plasmids are free from contaminating helper phage. This lab manual contains protocols for each of the techniques, but not strategies for how to use them (such as how to construct a restriction map, what to subclone, how to interpret BLAST results, etc). Not only will these strategies be specific for each plasmid studied, but we feel that students need to figure out these strategies on their own (with the instructors guidance, of course) to get the most they can out of the experience.

This fall, I have joined GCAT - the Genome Consortium for Active Teaching and we are pioloting Microarray Experiments as a part of the course work.  For more information on GCAT see http://www.bio.davidson.edu/Biology/GCAT/GCAT.html

Reprints of the paper mentioned on the home page of this manual are available on request from Jonathan Monroe. Comments are welcome!