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Week of: |
Activities |
Resources |
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Introduction |
GCAT Assessment |
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Martin Luther King Jr. Day No Class |
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January 25 |
RNA Isolation from Yeast Quiz on Protocol /Article (10 pts) |
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Making cDNA Quiz on Protocol (10 pts) |
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Microarray Hybridization and Washing (Note: some of this will take place outside of the regular class times ) Web Poster 1 Begun (10 pts) Dr. Slekar's Research Talk (Time to be Determined- 10 pts) |
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Microarray Analysis I - Gridding and Segmenting
Journal Club Presentations (20 pts) Dr. Jahan's Talk (Time To be Determined- 10 pts)
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Before Class take the time to look at the following: A short Movie on how your arrays were scanned: http://gcat.davidson.edu/sybr-u/scanning_at_davidson.mov Flash Tutorials On Microarray Analysis- Creating a New Project, Mouse Gridding V2.X and Segmentation Please Print out a Copy of: Scanalyze Guide Useful Links for Class: GCAT Information |
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Microarray Analysis II- Statistical Analysis and Beggining Array Interpretation Gridded Array Data Finished (10 pts) |
Before class take the time to look at the following: Stats Analysis Prepared by Alexa Romasco Stats Analysis Prepared by Charlie Cuccherini Flash Tutorials On Microarray Analysis- Expression Menu, Exploring, and Clustering Useful Links for Class: |
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Array Analyisis III - Intermediate Array Analysis and Individual Work Introduction to Project II Hypothesis for Project II (20 pts) Web Poster I (Intro, Methods, Results) (10 pts) |
Useful Links for Class: Stanford Microarray Database |
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March 8
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Spring Break No Class |
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March 15 |
Protein Extraction and Quantification Final Array Report Due March 22 (120 pts) |
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March 22 |
Western Blot - plan on 1 hour of work outside of the lab the day after your lab |
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Western Analysis Web Poster 2 Begun (10 pts) |
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DNA Extraction Web Poster 2 - (Intro- Methods- Results- 10 pts) |
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Real Time PCR Lab Exam Due (120 pts) |
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Interpretation of Real Time Results Corections on Array report Due (10 pts)
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Lab Wrap Up Final Rubisco Report Due April 30 (120 pts) |
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