Molecular Biology 480
Laboratory Schedule*
-Spring 2010-
*Note: Linked protocols for last half of this class and the background for this page are adapted from the GCAT website-- http://www.bio.davidson.edu/projects/gcat/gcat.html


Week of:
Scheduled
 Activities
Procedures and
Resources
January 11

Introduction
GCAT Assessment

Instructions for Array Web Poster
January 17

Martin Luther King Jr. Day

No Class

 
January 25

RNA Isolation from Yeast

Quiz on Protocol /Article (10 pts)

DeRisi Article

RNA Isolation and Analysis

RNA Quality Guide
February 1

Making cDNA

Quiz on Protocol (10 pts)

February 8

Microarray Hybridization and Washing

(Note: some of this will take place outside of the regular class times )

Web Poster 1 Begun (10 pts)

Dr. Slekar's Research Talk (Time to be Determined- 10 pts)

February 15

 

Microarray Analysis I - Gridding and Segmenting

 

Journal Club Presentations (20 pts)

Dr. Jahan's Talk (Time To be Determined- 10 pts)


Before Class take the time to look at the following:

A short Movie on how your arrays were scanned: http://gcat.davidson.edu/sybr-u/scanning_at_davidson.mov

Flash Tutorials On Microarray Analysis- Creating a New Project, Mouse Gridding V2.X and Segmentation

Please Print out a Copy of: Scanalyze Guide

Useful Links for Class:

GCAT Information
Analyzing your Array Picture
Your Gene List - go to yeast resources
Magic Tool Information
February 22

Microarray Analysis II-

Statistical Analysis and Beggining Array Interpretation

Gridded Array Data Finished (10 pts)

Before class take the time to look at the following:

Stats Analysis Prepared by Alexa Romasco

Stats Analysis Prepared by Charlie Cuccherini

Flash Tutorials On Microarray Analysis- Expression Menu, Exploring, and Clustering

Useful Links for Class:

Yeast Genomic Data Base
March 1

Array Analyisis III -

Intermediate Array Analysis and Individual Work

Introduction to Project II

Hypothesis for Project II (20 pts)

Web Poster I (Intro, Methods, Results) (10 pts)

Intro to Project 2

Useful Links for Class:

Microarray Global Viewer

Stanford Microarray Database

March 8

 

Spring Break

No Class

March 15

Protein Extraction and Quantification

Final Array Report Due March 22 (120 pts)

Protein Isolation

Web Poster 1 Instructions
March 22

Western Blot

- plan on 1 hour of work outside of the lab the day after your lab

Western Blot Protocol  

Antibody Detection
March 29

Western Analysis

Web Poster 2 Begun (10 pts)

Western Blot Protocol  

Antibody Detection
April 5

DNA Extraction

Web Poster 2 - (Intro- Methods- Results- 10 pts)

DNA Extraction
April 12

Real Time PCR

Lab Exam Due (120 pts)

Real Time PCR Protocol

Agarose Gel of Real time
April 19

 

Interpretation of Real Time Results

Corections on Array report Due (10 pts)

 


April 26

Lab Wrap Up

Final Rubisco Report Due April 30 (120 pts)

 

Go to Course Home Page

Dec. 19, 2010
T. Rife