Molecular Biology 480
Laboratory Schedule*
-Spring 2009-
*Note: Linked protocols for last half of this class and the background for this page are adapted from the GCAT website-- http://www.bio.davidson.edu/projects/gcat/gcat.html


Week of:
Scheduled
Activities
Procedures and
Resources
January 12

Introductions

Intro to Project 1
Intro to Project 2
GCAT Assessment
January 19

No Class MLK Day

Hypothesis- Due Wednesday, January 21 in Lecture

 
January 26

Protein Extraction/ Quantification

Protein Isolation
February 2

Western Blot

- plan on 1 hour of work outside of the lab the day after your lab

Western Blot Protocol  
February 9
Western Analysis

Antibody Detection

Web Poster 1 Instructions

February 16

DNA Extraction

DNA Extraction


February 23
Real Time PCR
Real Time PCR Protocol

March 2

 

Gel Analysis of Real Time PCR

Lab Exam Due Friday March 20
Web Poster Due Friday March 27

Agarose Gel of Real time

Guest Lectures - Week of March 2

An Overview of Microarray Data Analysis
March 9
No Lab Spring Break
 
March 16

RNA Isolation from Yeast

Microarray Paper Presentation Due- April 6

RNA Isolation and Analysis

RNA Quality Guide
Other Reading Material will be Posted on Blackboard

 

March 23
Making cDNA

Probe Construction

 

March 30

Hybridization and Washing (much of this will take place at alternative class times)

 

Hybridization

 

April 6

Array Analysis I - Gridding the Arrays

 

Before Class take the time to look at the following:

A short Movie on how your arrays were scanned: http://gcat.davidson.edu/sybr-u/scanning_at_davidson.mov

Flash Tutorials On Microarray Analysis- Creating a New Project, Mouse Gridding V2.X and Segmentation

Please Print out a Copy of: Scanalyze Guide

Useful Links for Class:

GCAT Information
Analyzing your Array Picture
Your Gene List - go to yeast resources
Magic Tool Information

DeRisi Article

April 13

Array Analysis II-

Statistical Analysis of your Array Data and Beginning Data Interpretation

Before class take the time to look at the following:

Stats Analysis Prepared by Alexa Romasco

Stats Analysis Prepared by Charlie Cuccherini

Flash Tutorials On Microarray Analysis- Expression Menu, Exploring, and Clustering

Useful Links for Class:

Yeast Genomic Data Base

A word document prepared by a former BIO 480 student on statistical analysis of arrays: StatisticalAnalysis.doc

April 20
Array Analysis III - Intermediate Data Interpretation and Individualized Work

 

Useful Links for Class:

Microarray Global Viewer

Stanford Microarray Database

April 27

Array Analysis IV -Individualized Work


Web Page II

Due Friday May 1 by 5 p.m.


GeneSpring Guide


Go to Course Home Page


Jan. 2, 2009
T. Rife