Microscopy Facility | Biology Department

Resources

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Cheat sheets for equipment in the microscopy facility

Spectra for different fluoreophores

Protocols for light microscopy

Software downloads

Links to online microscopy resources

Recommended books in the JMU Library

Cheat Sheets

Instructions for:

setting up DIC on the Nikon Eclipse

using wide field fluorescence on the Nikon Eclipse

starting up/shutting down the confocal

full instructions for confocal

Setting up DIC on the Zeiss Axioskop

Zeiss axioskop manual

Spectra for different fluorophores

Compare the excitation and emission spectra of multiple probes together with this Molecular Probes tool:

          http://probes.invitrogen.com/servlets/spectraviewer?fileid1=10436h2o

This one is pretty cool too:

http://www.mcb.arizona.edu/ipc/fret/index.html

Protocols for microscopy

Visit Invitrogen's online protocols guide

   Fixing whole Arabidopsis roots for immunolabelling of microtubules/DNA

Share your protocol here by emailing it to bannigax@jmu.edu

Software

Free image analysis software:

EZC1 Viewer - Free basic version of Nikon's confocal software

Image J - Free image processing software that can do almost anything you need (make sure you check out the Plugins)

Links

General Microscopy/Confocal Information

Molecular probes: http://probes.invitrogen.com/

If it's fluorescent, they've probably got it. There are also some excellent resources, including online tutorials.

Microscopy entry at Wikipedia. http://en.wikipedia.org/wiki/Microscopy

An excellent source of microscopy info.

      MicroscopyU: http://www.microscopyu.com/index.html

          Everything you ever wanted to know about light microscopy but were too lazy to ask

      Molecular Expressions: http://micro.magnet.fsu.edu/primer/index.html

          Similar to MicroscopyU

Zeiss - Microscopy from the beginning: http://www.zeiss.com/C1256B5E0047FF3F/?Open

General microscopy info and history of microscopy

      Microscopy UK: http://www.microscopy-uk.org.uk/mag/indexmag.html

          A fun site with photo galleries and handy DIY hints for adventurous microscopists

      For the physics-minded: http://www.physics.emory.edu/~weeks/confocal/

          How confocal microscopy works

Discussion Groups

Confocal Listserv: http://listserv.buffalo.edu/archives/confocal.html

A listserv for confocal users - good for getting obscure or very technical information about confocal microscopy

Journals & Magazines

Microscopy magazines: http://swehsc.pharmacy.arizona.edu/exppath/micro/freemags.html

This page has an extensive list of free microscopy magazines - both online and print - that you can subscribe to

Nature Reviews - focus on fluorescence imaging - http://www.nature.com/reviews/focus/fluorescence/index.html

Other microscopy facilities and Research Labs that specialize in microscopy:

Some of these other labs and facilities have some great info on microscopy and their research, as well as cool images.

Jim Hasseloff's Lab: http://www.plantsci.cam.ac.uk/Haseloff/

Confocal Facility at Ludwig Institute for Cancer Research: http://www.ludwig.edu.au/confocal/

WM Keck Laboratory for Biological Imaging: http://keck.bioimaging.wisc.edu/

Books

Murphy, Douglas B. (2001) Fundamentals of light microscopy and electronic imaging. Wiley-Liss (Library Call # QH211 .M87 2001)

Herman, B. (1997) Fluorescence Microscopy (2nd edition). Bios Scientific Publishers. (Library Call #: QH212.F55 H47 1998)

Pawley, James B. (2006) Handbook of Biological Confocal Microscopy (3rd edition). Springer. (Library Call #: QH224 .H36 2006 )

Microscopy Facility, Biology Department, James Madison University, Harrisonburg, VA